frozen human umbilical arterial endothelial cells Search Results


human umbilical artery endothelial cells  (PromoCell)
N/A
Primary Human Umbilical Endothelial Cells isolated from the arteries of the umbilical cord. Primary Human Umbilical Artery Endothelial Cells (HUAEC) are isolated from the arteries of the umbilical cord and are often used for in
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human umbilical artery endothelial cells  (Innoprot Inc)
N/A
Human Umbilical Artery Endothelial Cells
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umbilical artery huaec  (Cell Applications Inc)
94
Cell Applications Inc umbilical artery huaec
Umbilical Artery Huaec, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/frozen+human+umbilical+arterial+endothelial+cells/Human+Umbilical+Artery+Endothelial+Cells%3A+HUAEC/pm36383185-61-19-25
Average 94 stars, based on 1 article reviews
umbilical artery huaec - by Bioz Stars, 2026-09
94/100 stars
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human umbilical artery endothelial cells (huaec)  (ScienCell)
90
ScienCell human umbilical artery endothelial cells (huaec)
<t>Endothelial</t> ABCG1 expression was correlated with cholesterol efflux and intracellular lipid content. a, b HUAEC were transfected with scrambled siRNA, ABCG1 siRNA and either pcDNA (empty vector) or a pReceiver-ABCG1 expression vector. ABCG1 mRNA (a) and protein expression (b) were measured by real time PCR and western blotting. c Cholesterol efflux to HDL was decreased in ABCG1-deficient EC (ABCG1−), and reversed in ABCG1 overexpression plasmid transfection (ABCG1+). d Intracellular cholesterol content was increased in ABCG1-deficient EC while decreased in ABCG1 overexpression plasmid transfection. TC total cholesterol, FC free cholesterol, CE cholesterol ester. Data represent mean ± SD (n = 3). *P < 0.05, **P < 0.001 vs. EC control
Human Umbilical Artery Endothelial Cells (Huaec), supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/frozen+human+umbilical+arterial+endothelial+cells/human+umbilical+artery+endothelial+cells++huaec+/pmc10717156-42-0-8
Average 90 stars, based on 1 article reviews
human umbilical artery endothelial cells (huaec) - by Bioz Stars, 2026-09
90/100 stars
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human umbilical artery endothelial cells  (AcceGen Biotechnology)
N/A
AcceGen endothelial cells from large vessels are available from different locations, e.g. umbilical vein, umbilical artery, aorta, coronary artery, pulmonary artery, and saphenous vein. In addition, AcceGen offers HUVEC isolated in standard Endothelial Cell Growth
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gfp-human umbilical artery endothelial cells  (AcceGen Biotechnology)
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HUAECs are pooled cells isolated from normal human umbilical artery. GFP-HUAECs were selected from HUAECs infected with GFP-expressing lentiviruse with Puromycin and the cells are shipped in frozen vials (the cells are provided @ passage
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human umbilical artery endothelial cells  (Angio-Proteomie)
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rfp-human umbilical artery endothelial cells  (AcceGen Biotechnology)
N/A
HUAECs are pooled cells isolated from normal human umbilical artery. RFP-HUAECs were selected from HUAECs infected with RFP-expressing lentiviruses with Zyocin and the cells are shipped in frozen vials (the cells are provided @ passage
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rfp-human umbilical artery endothelial cells  (Angio-Proteomie)
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human umbilical artery endothelial cells (huaec), cell pellet in rnalater  (PromoCell)
N/A
Cell pellet of HUAEC from the arteries of the umbilical cord in RNAlater® for subsequent RNA, DNA or protein analysis. Cell pellet consisting of 1 million cells dissolved in 200 µl RNAlater® for subsequent RNA,
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human umbilical artery endothelial cell complete medium  (Elabscience Biotechnology)
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gfp-human umbilical artery endothelial cells  (Angio-Proteomie)
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Endothelial ABCG1 expression was correlated with cholesterol efflux and intracellular lipid content. a, b HUAEC were transfected with scrambled siRNA, ABCG1 siRNA and either pcDNA (empty vector) or a pReceiver-ABCG1 expression vector. ABCG1 mRNA (a) and protein expression (b) were measured by real time PCR and western blotting. c Cholesterol efflux to HDL was decreased in ABCG1-deficient EC (ABCG1−), and reversed in ABCG1 overexpression plasmid transfection (ABCG1+). d Intracellular cholesterol content was increased in ABCG1-deficient EC while decreased in ABCG1 overexpression plasmid transfection. TC total cholesterol, FC free cholesterol, CE cholesterol ester. Data represent mean ± SD (n = 3). *P < 0.05, **P < 0.001 vs. EC control

Journal: The Journal of Physiological Sciences : JPS

Article Title: ABCG1 deficiency promotes endothelial apoptosis by endoplasmic reticulum stress-dependent pathway

doi: 10.1007/s12576-013-0281-8

Figure Lengend Snippet: Endothelial ABCG1 expression was correlated with cholesterol efflux and intracellular lipid content. a, b HUAEC were transfected with scrambled siRNA, ABCG1 siRNA and either pcDNA (empty vector) or a pReceiver-ABCG1 expression vector. ABCG1 mRNA (a) and protein expression (b) were measured by real time PCR and western blotting. c Cholesterol efflux to HDL was decreased in ABCG1-deficient EC (ABCG1−), and reversed in ABCG1 overexpression plasmid transfection (ABCG1+). d Intracellular cholesterol content was increased in ABCG1-deficient EC while decreased in ABCG1 overexpression plasmid transfection. TC total cholesterol, FC free cholesterol, CE cholesterol ester. Data represent mean ± SD (n = 3). *P < 0.05, **P < 0.001 vs. EC control

Article Snippet: Cell culture Human umbilical artery endothelial cells (HUAEC; ScienCell) were cultured in Endothelial Cell Medium (No.1001; ScienCell) in a CO 2 /O 2 incubator at 37 °C.

Techniques: Expressing, Transfection, Plasmid Preparation, Real-time Polymerase Chain Reaction, Western Blot, Over Expression, Control

Downregulation of ABCG1 promoted endothelial apoptosis. Annexin V-FITC/PI double-staining assay was used to quantify apoptosis in HUAEC by means of flow cytometry. Endothelial apoptosis includes early apoptotic (FITC +PI−) and late apoptotic (FITC +PI+). a Apoptosis in endothelial control, b increased apoptosis in ABCG1-deficient EC, c apoptosis in ABCG1-deficient EC transfected with ABCG1 plasmid, d apoptosis in endothelial control transfected with ABCG1 plasmid

Journal: The Journal of Physiological Sciences : JPS

Article Title: ABCG1 deficiency promotes endothelial apoptosis by endoplasmic reticulum stress-dependent pathway

doi: 10.1007/s12576-013-0281-8

Figure Lengend Snippet: Downregulation of ABCG1 promoted endothelial apoptosis. Annexin V-FITC/PI double-staining assay was used to quantify apoptosis in HUAEC by means of flow cytometry. Endothelial apoptosis includes early apoptotic (FITC +PI−) and late apoptotic (FITC +PI+). a Apoptosis in endothelial control, b increased apoptosis in ABCG1-deficient EC, c apoptosis in ABCG1-deficient EC transfected with ABCG1 plasmid, d apoptosis in endothelial control transfected with ABCG1 plasmid

Article Snippet: Cell culture Human umbilical artery endothelial cells (HUAEC; ScienCell) were cultured in Endothelial Cell Medium (No.1001; ScienCell) in a CO 2 /O 2 incubator at 37 °C.

Techniques: Double Staining, Flow Cytometry, Control, Transfection, Plasmid Preparation

Blocking ER stress reduced endothelial apoptosis in ABCG1-deficient EC. Compared with control cells (a), endothelial apoptosis was significantly induced in ABCG1-deficient EC (b). c Endothelial apoptosis was partly reversed in ABCG1-deficient EC treated with PBA. d Endothelial apoptosis was moderately but not statistically decreased in control cells treated with PBA. Endothelial apoptosis includes early apoptotic (FITC +PI−) and late apoptotic (FITC +PI+) as described in “Methods”

Journal: The Journal of Physiological Sciences : JPS

Article Title: ABCG1 deficiency promotes endothelial apoptosis by endoplasmic reticulum stress-dependent pathway

doi: 10.1007/s12576-013-0281-8

Figure Lengend Snippet: Blocking ER stress reduced endothelial apoptosis in ABCG1-deficient EC. Compared with control cells (a), endothelial apoptosis was significantly induced in ABCG1-deficient EC (b). c Endothelial apoptosis was partly reversed in ABCG1-deficient EC treated with PBA. d Endothelial apoptosis was moderately but not statistically decreased in control cells treated with PBA. Endothelial apoptosis includes early apoptotic (FITC +PI−) and late apoptotic (FITC +PI+) as described in “Methods”

Article Snippet: Cell culture Human umbilical artery endothelial cells (HUAEC; ScienCell) were cultured in Endothelial Cell Medium (No.1001; ScienCell) in a CO 2 /O 2 incubator at 37 °C.

Techniques: Blocking Assay, Control

Cholesterol repletion and depletion were correlated with endothelial apoptosis and activation of ER stress. a Cyclodextrin (CD)-cholesterol loading of control cells increased FC and CE content. CD-cholesterol loading of ABCG1-deficient EC statistically increased CE content but not FC content. Cholesterol depletion with CD lowed FC and CE content in both endothelial control and ABCG1-deficient EC. b Cholesterol repletion induced endothelial apoptosis in endothelial controls. Cholesterol depletion significantly reversed endothelial apoptosis in ABCG1-deficient EC. Apoptosis was suppressed in ABCG1-deficient EC treated with U18666A, a cholesterol transport to ER inhibitor. c, d Cholesterol repletion increased the expression of GRP78 and CHOP, and cholesterol depletion reduced the expression of GRP78 and CHOP in ABCG1-deficient EC and control cells. e, f Addition of U18666A to ABCG1-deficient EC suppressed expression of GRP78 and CHOP. Data are mean ± SD (n = 3). *P = 0.05, **P < 0.001 vs. EC controls, # P < 0.001 vs. ABCG1-deficient EC. Representative images are shown for three independent experiments

Journal: The Journal of Physiological Sciences : JPS

Article Title: ABCG1 deficiency promotes endothelial apoptosis by endoplasmic reticulum stress-dependent pathway

doi: 10.1007/s12576-013-0281-8

Figure Lengend Snippet: Cholesterol repletion and depletion were correlated with endothelial apoptosis and activation of ER stress. a Cyclodextrin (CD)-cholesterol loading of control cells increased FC and CE content. CD-cholesterol loading of ABCG1-deficient EC statistically increased CE content but not FC content. Cholesterol depletion with CD lowed FC and CE content in both endothelial control and ABCG1-deficient EC. b Cholesterol repletion induced endothelial apoptosis in endothelial controls. Cholesterol depletion significantly reversed endothelial apoptosis in ABCG1-deficient EC. Apoptosis was suppressed in ABCG1-deficient EC treated with U18666A, a cholesterol transport to ER inhibitor. c, d Cholesterol repletion increased the expression of GRP78 and CHOP, and cholesterol depletion reduced the expression of GRP78 and CHOP in ABCG1-deficient EC and control cells. e, f Addition of U18666A to ABCG1-deficient EC suppressed expression of GRP78 and CHOP. Data are mean ± SD (n = 3). *P = 0.05, **P < 0.001 vs. EC controls, # P < 0.001 vs. ABCG1-deficient EC. Representative images are shown for three independent experiments

Article Snippet: Cell culture Human umbilical artery endothelial cells (HUAEC; ScienCell) were cultured in Endothelial Cell Medium (No.1001; ScienCell) in a CO 2 /O 2 incubator at 37 °C.

Techniques: Activation Assay, Control, Expressing